IncRNA Two-Cell Solutions

Dual-target Research Scheme of New Tumor Drug Target IncRNA

Based on TCGA big data, we analyzed and verified the new lncRNA functional genes that had not been reported in the studied tumors, and discussed the molecular mechanism of their pathogenesis, so as to provide a preliminary data basis for the determination of follow-up antineoplastic drug targets and biomarkers.

IncRNA Two-Cell Solutions

Long-chain non-coding RNA is a non-coding RNA with a length of more than 200bp. More and more studies have shown that lncRNA plays an important role in the occurrence and development of tumors. Through TCGA big data, we analyzed the high expression in cancer tissues or the expression of lncRNA, related to disease staging and grading. We analyzed the candidate lncRNA, that had not been reported in the studied tumors. Using lentivirus-mediated RNAi technology, several candidate lncRNA were RNAi, and the infected cells were counted for 4 to 5 days through the Cellomics high connotation function screening platform, and the target lncRNA, affecting cell proliferation was found. The intracellular localization of target lncRNA was determined by FISH experiment. At least 3 functional experiments of proliferative direction and 2 functional experiments of metastatic direction of the lncRNA in two cell lines were obtained. After interfering with the target lncRNA in a cell line, the transfer-related classical pathway combination panel was verified by WB. According to the results of WB panel, a positive mechanism gene was selected to construct a virus that interfered with or overexpressed the target lncRNA on the cell line, and interfered or overexpressed downstream mechanism genes on the cell line. The changes of cell proliferation ability were observed by HCS scanning for 5 days to verify the functional recovery effect of downstream mechanism genes on target lncRNA, and a positive recovery experiment results of proliferation-related and metastasis-related functional experiments were obtained.

IncRNA Two-Cell Solutions

Transfer-related Classical Pathway Combinations Panel

AKT1, p-AKT (Ser473 or Thr308), mTOR, p-mTOR (Ser2448), ERK1/2, p-ERK1/2 (Thr202+Tyr204), P38, p-P38 (T180+Y182), MYC, β-Catenin, p-β-Catenin (S33+S37), NFkB-p65, p-NFkB-p65 (Ser536), CDH1 (E-Cadherin), CDH2 (N-Cadherin), MMP2, MMP9, FN1, VIM, Snail, Slug, TWIST and other 22 genes.

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IncRNA Two-Cell Solutions

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Mature and reliable experimental analysis platform of Dual-target research scheme of new tumor drug target IncRNA: rich project experience, participation in major projects, high-level cooperation results published.

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IncRNA Two-Cell Solutions

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