Direct determination of DNA synthesis is one of the most accurate methods to detect tumor cell proliferation, and it is also a basic method to determine substance toxicity, drug safety evaluation and cell health. At present, the commonly used tumor cell proliferation detection methods include thymine nucleoside (3H-TdR) infiltration method, MTT detection, hydroxyfluorescein diacetate succinimide (CFSE) detection, Brdu detection and EDU detection. We have high-quality researchers and experienced teams to provide you with personalized experimental solutions related to tumor cell proliferation. With the most accurate experimental results to help you better carry out cancer-related scientific research.
Figure 1. CCK-8 detection schematic diagram
Cell Counting Kit, abbreviated as CCK-8, is a rapid and highly sensitive detection kit widely used in cell proliferation and cytotoxicity. In the presence of electron coupling reagents, WST-8 can be reduced by dehydrogenase in the mitochondria to a highly water-soluble yellow nail product. The depth of color is directly proportional to cell proliferation and inversely proportional to cytotoxicity. The OD value at the wavelength of 450nm was measured by enzyme labeling instrument, which indirectly reflected the number of living cells.
MTT method, also known as MTT colorimetric method, is a method to detect cell survival and growth. The detection principle is that succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-purple crystal methylazan and deposit in the cells, while dead cells do not have this function. Dimethyl sulfoxide can dissolve methylazan in cells, and its light absorption value is measured at 540 or 720nm wavelength by enzyme-linked immunosorbent assay (Elisa), which can indirectly reflect the number of living cells. In a certain range of cell number, the amount of MTT crystallization is proportional to the number of cells.
Figure 2. MTT ASSAY (Cell Viability Assay)
Scope of application
MTT method has been widely used in the activity detection of some bioactive factors, large-scale screening of antineoplastic drugs, cytotoxicity test and tumor radiosensitivity test.
At present, CCK-8 has been widely used in high-throughput drug screening, cell proliferation test, cytotoxicity test, tumor drug sensitivity test and biological factor activity test and so on.
Provided by the customer
1. The tumor cells to be tested need to be transported by dry ice or liquid nitrogen after cryopreservation, or can be sent at room temperature after resuscitation.
2. The drug or protein samples to be tested need to be transported by dry ice, and the freeze-dried samples can be sent at 4℃.
The original experimental data, analysis results, experimental flow and a complete report.
10-20 working days;
Figure 3. Result display
Mature and reliable experimental analysis platform of tumor cell proliferation: rich project experience, participation in major projects, high-level cooperation results published.
Based on high-quality data analysis, pre-research and verification of the contents and methods of analysis, it is indeed mature and feasible.
Creative Proteomics leads a rich team that uses the most rigorous experimental solutions and internationally recognized tumor cell proliferation experimental techniques to obtain your customized information. Use the most accurate experimental results to help you better carry out tumor-related scientific research.
Please contact us to find out how we can help you achieve the next research breakthrough.