There are practical or potentially valuable cancer single cell research methods in cancer research, including single cell isolation, whole genome and transcriptome amplification, epigenome analysis, multidimensional sequencing and next generation sequencing and analysis. Single-cell sequencing includes a set of powerful and comprehensive techniques that provide an in-depth understanding of these unknown objects. Advances in genomic technology have enabled people to detect mutations and gene expression profiles of cancer cells at the single-cell level. Single cell sequencing can make a comprehensive and accurate analysis of cancer cell genomes, transcriptome and epigenome. The use of single cell sequencing analysis of cancer cells can directly see the high heterogeneity and random changes in the cancer cell population, new driver mutations and complex clonal evolution mechanisms, as well as the identification of new biomarkers. Single cell sequencing methods include single cell isolation, whole genome and transcriptome amplification, epigenome analysis, multidimensional sequencing and next generation sequencing and analysis.
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